| Solution Information | help | |
| Enzyme: | Group 3 secretory phospholipase A2 | |
| inhibitor: | BDBM49612 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose responses curves for compounds identified as active in a previous set of experiments entitled, "QFRET-based biochemical high throughput primary assay to identify inhibitors of human group III secreted phospholipase A2 enzyme (HGIII-sPLA2)" (PubChem AID 743126) and "QFRET-based biochemical high throughput confirmation assay to identify inhibitors of human group III secreted phospholipase A2 enzyme (HGIII-sPLA2) (PubChem AID 743280) and were inactive in a set of experiments entitled, "Counterscreen for inhibitors of the HGIII-SPLA2: QFRET-based biochemical high throughput screening assay to identify inhibitors that optically interfere with 535nm fluorescence (PubChem AID 743282). In this assay, HGIII-sPLA2 enzyme is pre-incubated with test compounds, followed by incubation with the BODIPY PC-A2 substrate in a suspension of DOPC:DOPG liposomes. Fluorescence is determined at at specific time point. The cleavage of the BODIPY | |
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